kinase_conservation_analysis.py Command Help
Command: $SCHRODINGER/run -FROM psp kinase_conservation_analysis.py
usage: kinase_conservation_analysis.py [-h] [-distance_cutoff DISTANCE_CUTOFF]
-ligand_asl LIGAND_ASL -protein_db
{kinase,nhr,gpcr}
[-angle_cutoff ANGLE_CUTOFF]
-receptor_chain RECEPTOR_CHAIN [-pval]
[-groups {TKL,RGC,AGC,CMGC,STE,OTHER,TK,CAMK,CK1} [{TKL,RGC,AGC,CMGC,STE,OTHER,TK,CAMK,CK1} ...]]
[-jobname JOBNAME] [-HOST <hostname>]
[-WAIT] [-D] [-NOJOBID] [-NJOBS NJOBS]
[-RETRIES RETRIES] [-NOLAUNCH]
query_structure
Given a holo structure, annotates its protein sequence with ligand binding
residues within a given distance cuttoff (7.0 Angstrom by default). Then uses
BLOSUM matrix to calculate a position specific conservation score of the
ligand binding residues.
positional arguments:
query_structure A target ligand/receptor (holo) complex structure.
options:
-h, --help show this help message and exit
-distance_cutoff DISTANCE_CUTOFF
A distance cutoff from ligand to collect residues on
receptor protein (default: 7.0)
-ligand_asl LIGAND_ASL
ASL defining the ligand atoms (default: None)
-protein_db {kinase,nhr,gpcr}
Specify the protein database for conservation
analysis.
-angle_cutoff ANGLE_CUTOFF
A side chain angle cutoff to determine if a residue is
pointing to the ligand (default: 105.0)
-receptor_chain RECEPTOR_CHAIN
A chain ID of the receptor
-pval A flag to print p-values and associated z-scores in
.csv file
-groups {TKL,RGC,AGC,CMGC,STE,OTHER,TK,CAMK,CK1} [{TKL,RGC,AGC,CMGC,STE,OTHER,TK,CAMK,CK1} ...]
Run conservation analysis within selected groups.
-jobname JOBNAME, -JOBNAME JOBNAME, -j JOBNAME
Set the base name of outputs
Job Control Options:
-HOST <hostname> Run job remotely on the indicated host entry.
-WAIT Do not return a prompt until the job completes.
-D, -DEBUG Show details of Job Control operation.
-NOJOBID Run the job directly, without Job Control layer.
Standard Options:
-NJOBS NJOBS Divide the overall job into NJOBS subjobs.
-RETRIES RETRIES If a subjob fails for any reason, it will be retried
RETRIES times. (Default: 2)
-NOLAUNCH Set up subjob inputs, but don't run the jobs.